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An improved method to distinguish micrococcal nuclease sensitivity of chromatin
Q A Hamid1, S Thanumalayan, V K Parnaik
1Centre for Cellular and Molecular Biology, Hyderabad, India.
Journal of Biochemical and Biophysical Methods
|October 15, 1996
Abstract:
The resolution of the standard micrococcal nuclease assay for sensitivity of active chromatin has been enhanced by the inclusion of an additional step of digesting nuclease-digested DNA with a suitable restriction enzyme prior to Southern hybridization. The improved assay has been used to analyze the chromatin structure of the lamin A, albumin and alpha-fetoprotein genes during rat liver development.