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Folding and aggregation of DNA chains induced by complexation with lipospermine: formation of a nucleosome-like
1Graduate School of Human Informatics, Nagoya University, Japan.
FEBS Letters
|October 28, 1996
Abstract:
Dioctadecylamidoglycylspermine (DOGS) is a cationic lipid vector capable of efficiently introducing DNA into various eukaryotic cells. We investigated the higher-order structure of the DNA/DOGS complex using fluorescence and electron microscopy. Our results show that the DNA/DOGS complex exhibits a nucleosome-like structure in which DNA wraps around an aggregate of DOGS molecules. In addition, DNA/DOGS complexes tend to associate with each other to form network structures. The resulting network assembly may play a role in effective gene transfection.