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Published on: June 29, 2016
Intracellular adhesion molecule-1 and lymphocyte function-associated antigen-1 expression in a rat forebrain
1Department of Neurosurgery, Hokkaido University School of Medicine, Sapporo.
Neurologia Medico-Chirurgica
|February 1, 1996
Summary
Activated leukocytes contribute to brain reperfusion injury. Increased expression of intracellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1) on brain vessels and leukocytes, respectively, are observed following reperfusion.
Area of Science:
- Neuroscience
- Immunology
- Pathology
Background:
- Activated leukocytes are implicated in brain reperfusion injury.
- Intracellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1) mediate leukocyte-brain interactions.
Purpose of the Study:
- To investigate the expression of ICAM-1 in cerebral vessels and LFA-1 on leukocytes after reperfusion in a rat model.
- To understand the role of these molecules in the pathophysiology of brain reperfusion injury.
Main Methods:
- A rat four-vessel occlusion model was used to induce forebrain ischemia followed by reperfusion.
- Immunohistological staining was performed to detect ICAM-1 and LFA-1 expression at various reperfusion time points (15 min to 24 hrs).
Main Results:
- ICAM-1 expression increased significantly after 1 hour of reperfusion and remained elevated on cerebral microvessels.
- LFA-1-positive leukocytes were detected in cerebral capillaries by 6 hours of reperfusion.
- Reperfusion induced increased expression of both ICAM-1 and LFA-1.
Conclusions:
- The study demonstrates that reperfusion upregulates ICAM-1 and LFA-1 expression in the rat brain.
- These molecular changes suggest a significant role for leukocyte adhesion molecules in the development of brain reperfusion injury.
