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Updated: Aug 2, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of nitrite and
A Csiba1, M Szentgyörgyi, S Juhász
1Veterinary and Food Control Station of Capital Budapest, Hungary.
Abstract:
A high-performance liquid chromatographic (HPLC) procedure with fluorometric detector has been developed for the determination of nitrite and formaldehyde from foods by the use of hydralazine. Hydralazine reacts with nitrite and formaldehyde under acidic conditions in boiling water-bath for 15 min to form tetrazolo-(5,1-a)-phthalazine (Tetra-P) and triazolo-(3,4-a)-phthalazine (Tri-P) quantitatively. Without extraction, the determination of Tetra-P and Tri-P was simple, specific, sensitive and reliable over the range of 0.003-0.3 ppm of sodium nitrite and 0.02-0.4 ppm of formaldehyde. This procedure using hydralazine is one of the most useful methods for routine analysis of nitrite and formaldehyde in foods, biological fluids and ambient waters.
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