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Seizures induce tenascin-C mRNA expression in neurons
L Ferhat1, N Chevassus-Au-Louis, M Khrestchatisky
1Université René Descartes, Paris V, INSERM U29, France.
Journal of Neurocytology
|September 1, 1996
Summary
Kainate-induced seizures increase tenascin-C (an extracellular matrix glycoprotein) expression in rat brains, with both neurons and glia producing it rapidly. This suggests a role in early post-seizure brain responses.
Area of Science:
- Neuroscience
- Cell Biology
- Biochemistry
Background:
- Tenascin-C is an extracellular matrix glycoprotein with dual roles in neural development and repair.
- Astrocytes are the primary producers of Tenascin-C in the central nervous system (CNS).
Purpose of the Study:
- To investigate the expression and cellular sources of Tenascin-C following kainate-induced seizures in the rat brain.
- To determine the temporal dynamics of Tenascin-C induction in response to seizures.
Main Methods:
- Kainate administration to induce seizures in rats.
- Quantitative analysis of Tenascin-C mRNA expression using in situ hybridization.
- Double labeling experiments with Tenascin-C probes and neuronal marker MAP2.
Main Results:
- Kainate-induced seizures significantly increased Tenascin-C mRNA expression in the hippocampus, pyriform cortex, and amygdala.
- Tenascin-C mRNA was detected in neurons, particularly in the granule cell layer, and also in glial cells.
- Seizure-induced Tenascin-C expression was rapid, observed within 6 hours, preceding glial reactions or lesions.
Conclusions:
- Tenascin-C is rapidly upregulated in the rat brain following seizures.
- Both neurons and glia contribute to Tenascin-C production in the early stages of seizure pathology.
- Tenascin-C may play a role in the immediate cellular responses to seizures, potentially influencing scarring and remodeling.