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Sialyl Lewis(x)/E-selectin-mediated rolling in a cell-free system
D K Brunk1, D J Goetz, D A Hammer
1School of Chemical Engineering, Cornell University, Ithaca, New York 14853, USA.
Biophysical Journal
|November 1, 1996
Summary
This study shows that sialyl Lewis(x) is essential for neutrophil rolling on E-selectin during inflammation. The cell-free assay confirms selectin-carbohydrate bonds drive initial adhesion under physiological flow conditions.
Area of Science:
- Immunology
- Cell Biology
- Biophysics
Background:
- Selectins mediate transient adhesion of neutrophils to endothelial cells at inflammatory sites.
- This adhesion involves carbohydrate-binding interactions, such as sialyl Lewis(x) with E-selectin.
Purpose of the Study:
- To investigate the role of selectin-carbohydrate bond properties in neutrophil rolling.
- To determine if cellular morphology and signaling are primary or secondary factors in rolling adhesion.
Main Methods:
- Development of a cell-free adhesion assay using sialyl Lewis(x)-coated microspheres.
- Utilizing E-selectin-IgG chimera-coated substrates to mimic endothelial cells.
- Measuring rolling velocities under physiological wall shear stresses (0.7–2 dynes/cm²).
Main Results:
- Sialyl Lewis(x)-coated microspheres demonstrated attachment and rolling on E-selectin-coated substrates.
- Rolling velocities were dependent on time, E-selectin site density, and wall shear stress.
- Confirmed sialyl Lewis(x) as a minimal recognition element for E-selectin-mediated rolling.
Conclusions:
- Selectin-carbohydrate bond properties are the primary drivers of neutrophil rolling adhesion.
- Cellular morphology and signaling play secondary roles in this adhesion process.
- The findings support a model where specific molecular interactions govern initial cell adhesion under flow.