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Related Experiment Videos

Progressive decrease in VH3 gene family expression in plasma cells of HIV-infected patients

D David1, C Demaison, L Bani

  • 1Départment d'Immunologie, Institut Pasteur, Paris, France.

International Immunology
|August 1, 1996
PubMed
Summary
This summary is machine-generated.

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In HIV-infected patients, the VH3 gene family expression is significantly reduced in plasma cells, especially in those with AIDS. This VH3 gene reduction may contribute to weakened immune responses against bacterial infections.

Area of Science:

  • Immunology
  • Virology
  • Molecular Biology

Background:

  • Human Immunodeficiency Virus (HIV) infection is known to affect B lymphocyte populations.
  • Previous studies indicated alterations in VH gene family expression within peripheral B lymphocytes during HIV infection.

Purpose of the Study:

  • To investigate the expression levels of VH gene families in peripheral plasma cells of HIV-infected individuals.
  • To determine if VH gene family expression in plasma cells is altered compared to healthy donors and correlates with disease progression.

Main Methods:

  • Analysis of VH gene family expression in peripheral plasma cells (mu and gamma isotypes) from HIV-infected patients and healthy donors.
  • Separation of CD19+ and CD20+ B lymphocytes.
  • Anchored RT-PCR followed by hybridization with specific VH gene family and JH region probes.

Related Experiment Videos

  • Quantification of VH expression using VH/JH hybridization intensity ratios.

    • Main Results:

    • A significant reduction in VH3 gene family expression was observed in plasma cells of all HIV-infected patients compared to healthy controls.
    • This VH3 gene expression decrease was most pronounced in patients with Acquired Immunodeficiency Syndrome (AIDS), exceeding 90%.
    • Expression levels of VH1 and VH4 gene families remained largely unchanged in HIV-infected patients.

    Conclusions:

    • VH3 gene family expression is demonstrably affected in plasma cells during HIV infection, extending previous findings in B lymphocytes.
    • The observed reduction in VH3 expression may be linked to a B cell superantigen associated with HIV.
    • Impaired humoral immunity against bacterial pathogens in HIV-infected individuals could be partly attributed to this pronounced decrease in VH3 gene family expression.