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Quantification of Bcr-Abl transcripts in chronic myelogenous leukemia (CML) using standardized, internally
1Abteilung für Innere Medizin m.S. Hämatologie und Onkologie, Virchow Klinikum, Medizinische Fakultät der Humboldt Universität zu Berlin, Germany.
Nucleic Acids Research
|October 15, 1996
Abstract:
The quantification of Bcr-Abl transcript numbers in chronic myelogenous leukemia (CML) patients described here uses simultaneous competitive PCR amplification of the target gene (Bcr-Abl) and a reference gene (porphobilinogen deaminase; Pbgd) together with a single composite competitor molecule for both targets based on heterologous sequences. Using this technique, Bcr-Abl transcript numbers could be reproducibly determined even in clinical samples known to harbour poor quality RNA.