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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

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An improved method for the detection of Tospoviruses using the polymerase chain reaction

R A Mumford1, I Barker, K R Wood

  • 1School of Biological Sciences, University of Birmingham, UK.

Journal of Virological Methods
|March 1, 1996
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...