Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

AP-1 DNA-binding activation by methamphetamine involves oxidative stress

P Sheng1, X B Wang, B Ladenheim

  • 1Section of Molecular Neuropsychiatry, Intramural Research Program, NIH/NIDA, Baltimore, Maryland 21224, USA.

Synapse (New York, N.Y.)
|November 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Efficacy and safety of fourth-generation CAR-T cells targeting CD19 and BCMA with anti-IL-6 antibody secretion in refractory systemic lupus erythematosus].

Zhonghua yi xue za zhi·2026
Same author

A versatile two-dimensional terahertz spectroscopy platform with dual independently controlled intense pulses.

The Review of scientific instruments·2026
Same author

[Analysis of the prevalence status and clinical characteristics of the hepatitis D virus in the Xinjiang region].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2025
Same author

[Impact of cervical diffuse idiopathic skeletal hyperostosis on dysphagia and its surgical management].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2025
Same author

[Exploring the advantages of traditional Chinese medicine and dual-anti therapy for liver cirrhosis].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2025
Same author

[Study on the efficacy and safety profile of tenofovir alafenamide fumarate in the treatment of patients with decompensated hepatitis B cirrhosis].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2025
Same journal

LncRNA MIAT Protects Against Sevoflurane-Induced Cognitive Dysfunction in Neonatal Rats via the miR-15b-5p/Ccnd1 Axis.

Synapse (New York, N.Y.)·2026
Same journal

A Survey of Automatic Sleep Staging: Technical Route, Technical Challenges, and Future Perspectives.

Synapse (New York, N.Y.)·2026
Same journal

Structural Architecture and Evolutionary Conservation of Cerebellin-Mediated Trans-Synaptic Signaling.

Synapse (New York, N.Y.)·2026
Same journal

Correction to "Time of Day Does Not Impact Spinal Serotonin Levels in Humans".

Synapse (New York, N.Y.)·2026
Same journal

Effect of Healthy Aging on the Cytoarchitecture of Layer V Pyramidal Neurons in the Motor Cortex of Rats: A Golgi Study.

Synapse (New York, N.Y.)·2026
Same journal

Ketamine Responsiveness of Mice Exhibiting Anorexia-Like Behavior Correlates With Synaptic AMPA Receptor Distribution.

Synapse (New York, N.Y.)·2026
See all related articles

Methamphetamine (METH) increases AP-1 DNA-binding activity in mice, with effects dependent on cellular redox status. Transgenic mice with enhanced antioxidant enzymes showed reduced METH-induced AP-1 activation.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Methamphetamine (METH) is a potent psychostimulant with known neurotoxic effects.
  • Activating transcription factor-1 (AP-1) is a crucial transcription factor involved in cellular responses to stress and stimuli.
  • Cellular redox status plays a significant role in regulating gene expression and transcription factor activity.

Purpose of the Study:

  • To investigate the impact of METH on AP-1 DNA-binding activity in vivo.
  • To determine the influence of cellular redox status, specifically via CuZn-SOD, on METH-induced AP-1 activation.
  • To explore the time-course and regional distribution of METH's effects on AP-1.

Main Methods:

  • Dose-dependent administration of METH to nontransgenic (Non-Tg) and copper-zinc superoxide dismutase transgenic (SOD-Tg) mice.

Related Experiment Videos

  • Measurement of AP-1 DNA-binding activity using established biochemical assays.
  • Analysis of AP-1 activity in specific brain regions (caudate putamen, cerebellum, striatum) over a 72-hour period.

    • Main Results:

    • METH administration led to dose-dependent increases in AP-1 DNA-binding activity in both Non-Tg and SOD-Tg mice.
    • AP-1 activity peaked at 3 hours post-METH administration and returned to baseline by 72 hours.
    • SOD-Tg mice exhibited attenuated increases in AP-1 DNA-binding activity compared to Non-Tg mice, particularly in the striatum.

    Conclusions:

    • METH-induced stimulation of AP-1 DNA-binding activity is dependent on the cellular redox state.
    • Enhanced antioxidant capacity, as seen in SOD-Tg mice, can mitigate METH's effects on AP-1.
    • These findings support the role of redox mechanisms in regulating transcription factors like AP-1 in response to METH exposure.