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A new method for permeabilization of cultured cells without cell damage

N Shimizu1, Y Kawazoe

  • 1Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.

Biological & Pharmaceutical Bulletin
|March 1, 1996
PubMed
Summary

High molecular weight polyacrylic acid (PAA) transiently permeabilizes mammalian cells via vortex-stirring. This method enhances uptake of non-permeant molecules like Lucifer Yellow and increases bleomycin cytotoxicity in leukemia cells.

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Area of Science:

  • Cell biology
  • Biotechnology
  • Drug delivery

Background:

  • Mammalian cell membrane integrity is crucial for cellular function.
  • Introducing molecules into cells typically requires specific delivery methods.
  • Developing efficient transient permeabilization techniques is vital for research and therapeutics.

Purpose of the Study:

  • To investigate a novel method for transiently permeabilizing cultured mammalian cells.
  • To assess the efficacy of polyacrylic acid (PAA) and vortex-stirring for cell membrane permeabilization.
  • To evaluate the impact of this permeabilization on the uptake of non-permeant molecules and drug cytotoxicity.

Main Methods:

  • Cultured mammalian cells (murine leukemia L1210) were subjected to vortex-stirring.

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  • High molecular weight polyacrylic acid (PAA) at concentrations of 1-10 µg/ml was used.
  • Lucifer Yellow (LY) uptake and bleomycin (BLM) cytotoxicity were measured to assess permeabilization.

    • Main Results:

    • Transient cell permeabilization was achieved within seconds using PAA and vortex-stirring.
    • Significant uptake of the non-permeant dye Lucifer Yellow (LY) was observed in treated cells.
    • The cytotoxicity of bleomycin (BLM), a poor permeant, was markedly enhanced by this procedure.

    Conclusions:

    • Vortex-stirring with high molecular weight polyacrylic acid provides an effective method for transiently permeabilizing mammalian cells.
    • This technique facilitates the entry of otherwise non-permeant molecules into cells.
    • The enhanced permeabilization shows potential for improving drug delivery and efficacy, particularly for poorly permeant chemotherapeutics.