Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simplified method for measuring urinary leukotriene E4

D W Qiu1, K P Hui, C W Lee

  • 1Department of Medicine, National University of Singapore, Singapore.

Journal of Chromatography. B, Biomedical Applications
|February 23, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Estimation of standardized ileal digestible calcium and phosphorus requirements of broiler chickens aged 21-35 days.

Poultry science·2025
Same author

Development and psychometric validation of the Chronic Rhinosinusitis Control Test.

Rhinology·2025
Same author

Correction: PPARγ is essential for protection against nonalcoholic steatohepatitis.

Gene therapy·2025
Same author

Efficacy and safety of home-based transcranial direct current stimulation as adjunct treatment for cognitive improvement in major depressive disorder: A double-blind, randomized, multi-site clinical trial.

European psychiatry : the journal of the Association of European Psychiatrists·2025
Same author

Utility of cranial MRI in non-traumatic headache patients with prior negative head CT within 1 month.

Clinical radiology·2023
Same author

Low Intake of Choline Is Associated with Diminished Strength and Lean Mass Gains in Older Adults.

The Journal of frailty & aging·2023
Same journal

Validated assay for the determination of celiprolol in plasma using high-performance liquid chromatography and a silanol deactivated reversed-phase support.

Journal of chromatography. B, Biomedical applications·1996
Same journal

High-performance chromatographic assay for the sulphoxide metabolite of 2'-deoxy-3'-thiacytidine in human urine.

Journal of chromatography. B, Biomedical applications·1996
Same journal

Rapid method for the analysis of itraconazole and hydroxyitraconazole in serum by high-performance liquid chromatography.

Journal of chromatography. B, Biomedical applications·1996
Same journal

Micropreparation of hemopoietic stem cells from the mouse bone marrow suspension by gravitational field-flow fractionation.

Journal of chromatography. B, Biomedical applications·1996
Same journal

Evidence of metal binding activities of pentadecapeptide from Panax ginseng.

Journal of chromatography. B, Biomedical applications·1996
Same journal

Determination of bezafibrate, ciprofibrate and fenofibric acid in human plasma by high-performance liquid chromatography.

Journal of chromatography. B, Biomedical applications·1996
See all related articles

Measuring urinary leukotriene E4 (LTE4) can be simplified. Enzyme immunoassay (EIA) alone, after crude extraction, shows strong correlation with HPLC-purified samples, suggesting it

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Immunology

Background:

  • Urinary leukotriene E4 (LTE4) is a key biomarker in inflammatory and allergic conditions.
  • Conventional measurement involves reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with radioimmunoassay (RIA) or enzyme immunoassay (EIA).
  • This method is time-consuming and resource-intensive for routine clinical use.

Purpose of the Study:

  • To evaluate the necessity of HPLC purification for routine urinary LTE4 measurement.
  • To compare urinary LTE4 levels measured by EIA alone versus HPLC-purified EIA.
  • To assess the clinical applicability of a simplified LTE4 assay.

Main Methods:

  • Ninety-three urine samples from normal subjects and patients with bronchial asthma and adult respiratory distress syndrome were analyzed.

Related Experiment Videos

  • Urinary LTE4 was measured using two methods: EIA alone after crude extraction (Sep-Pak cartridge) and HPLC followed by EIA.
  • Correlation analysis was performed to compare results from both methods.

    • Main Results:

    • Urinary LTE4 levels measured by EIA alone showed significant correlation with those measured by HPLC plus EIA across all groups (r = 0.88, 0.85, 0.68).
    • Absolute urinary LTE4 values obtained by EIA alone were higher than those obtained by HPLC-purified EIA.
    • No significant differences in correlation were observed between patient groups and normal subjects.

    Conclusions:

    • HPLC purification may not be essential for routine urinary LTE4 quantitation in various clinical settings.
    • EIA alone, following crude urine extraction, provides a reliable and potentially more efficient method for LTE4 measurement.
    • This simplified approach could facilitate broader clinical application of urinary LTE4 analysis.