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[Effects of some detergents in measuring inorganic phosphorus: a comparative characterization of 2 methods]
Klinicheskaia Laboratornaia Diagnostika
|May 1, 1996
Abstract:
The authors compare the effects of Tween-20, Brij-35, Triton X-100 and X-305 on the development of stain for two methods: (1) with phosphoric molybdenic acid forming a complex with malachite blue and (2) with photometry of nonreduced phosphoric molybdenic complex. In the former method the type of detergent affects the degree of absorption of the photo-measured complex and in the latter it affects only the duration of the complex stabilization. Both methods are sufficiently sensitive, rapid, and reproducible. In both cases Triton X-100 was used in routine studies.