Preparation and characterization of aflatox-n B1-1-(O-carboxymethyl) oxime

A method is described for the preparation and purification of aflatoxin B1-1(O-carboxymethyl) oxime from aflatoxin B1. The overall yield was about 73-83%. The new aflatoxin B1 derivative was characterized by mass, ultraviolet, infrared, and nuclear magnetic resonance spectral analyses, and was nontoxic to 8-day-old chicken embryos when tested at a concentration of 3.48 microgram/egg.