Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Migration01:09

Cell Migration

16.6K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
16.6K
Determination01:51

Determination

16.3K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
16.3K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

8.6K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.6K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

6.4K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
6.4K
Cell Migration01:19

Cell Migration

6.1K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.1K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.1K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnosis and management of acquired aplastic anemia in childhood. Guidelines from the Marrow Failure Study Group of the Pediatric Haemato-Oncology Italian Association (AIEOP).

Blood cells, molecules & diseases·2024
Same author

Acute chest syndrome in children with sickle cell disease: Data from a national AIEOP cohort identify priority areas of intervention in a hub-and-spoke system.

British journal of haematology·2023
Same author

Protective effect of Aquaphilus dolomiae extract-G1, ADE-G1, on tight junction barrier function in a Staphylococcus aureus-infected atopic dermatitis model.

Journal of the European Academy of Dermatology and Venereology : JEADV·2020
Same author

A Case Series and Literature Review of Isavuconazole Use in Pediatric Patients with Hemato-oncologic Diseases and Hematopoietic Stem Cell Transplantation.

Antimicrobial agents and chemotherapy·2019
Same author

Control of Multifunctional Prosthetic Hands by Processing the Electromyographic Signal.

Critical reviews in biomedical engineering·2018
Same author

Development of a portable sensorised handle for the objective assessment of the effectiveness and concordance of intervention plans in dementia.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2017

Related Experiment Video

Updated: May 4, 2026

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

10.2K

Direct and long-range action of a wingless morphogen gradient

M Zecca1, K Basler, G Struhl

  • 1Zoologisches Institut der Universität Zürich, Switzerland.

Cell
|November 29, 1996
PubMed
Summary

Wingless (Wg) acts as a long-range morphogen in Drosophila development by directly regulating gene transcription based on concentration and distance. This contrasts with tethered Wg or Armadillo (Arm) which have limited or autonomous effects.

More Related Videos

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
11:48

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Published on: January 17, 2016

8.3K
Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages
08:25

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Published on: June 2, 2020

8.6K

Related Experiment Videos

Last Updated: May 4, 2026

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

10.2K
Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
11:48

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Published on: January 17, 2016

8.3K
Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages
08:25

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Published on: June 2, 2020

8.6K

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Wingless (Wg) is a secreted protein crucial for Drosophila development.
  • Wg signaling involves the cytosolic protein Armadillo (Arm).
  • Wg's precise mechanism of action, particularly its range, is under investigation.

Purpose of the Study:

  • To investigate the effects of wild-type Wg, membrane-tethered Wg, and constitutively active Armadillo (Arm) on gene transcription.
  • To compare these effects with the removal of endogenous Wg or Arm activity.
  • To elucidate the role of Wg as a long-range morphogen.

Main Methods:

  • Ectopic expression of wild-type Wg, membrane-tethered Wg, and constitutively active Arm.
  • Comparison with the effects of removing endogenous Wg or Arm activity.
  • Analysis of target gene transcription in response to Wg signaling.

Main Results:

  • Wild-type Wg up-regulates target gene transcription in a concentration- and distance-dependent manner, indicating long-range action.
  • Membrane-tethered Wg exhibits short-range effects on gene transcription.
  • Constitutively active Arm shows autonomous effects on gene transcription.

Conclusions:

  • Wg functions as a direct, long-range gradient morphogen during Drosophila development.
  • The range of Wg signaling is dependent on its secreted nature.
  • Armadillo's role in Wg transduction is essential but distinct from Wg's long-range morphogenetic activity.