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Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
Published on: December 26, 2017
Substance P primes murine peritoneal macrophages for an augmented proinflammatory cytokine response to
A S Berman1, C Chancellor-Freeland, G Zhu
1Department of Microbiology, Boston University School of Medicine, Mass 02118, USA.
Abstract:
We have recently shown that substance P (SP) participates in the stress-induced modulation of elicited, peritoneal macrophage function. This study reports the in vitro effects of SP on macrophage activity. We show by an MTT bioassay that SP significantly increases cellular metabolic activity. We show by ELISA that preincubating (priming) the macrophages with SP, prior to the incubation with lipopolysaccharide (LPS), results in a significant enhancement of proinflammatory cytokine secretion, relative to LPS alone. Finally, we show that somatostatin can antagonize the SP-induced enhancement of cytokine secretion. The above results demonstrate the importance of the temporal sequence in which stimuli are administered, in vitro, and indicate that SP can act as first signal in the cascade of macrophage activation. We postulate that stress, via the secretion of SP and other sensory neuropeptides, may play a role in the pathogenesis of certain inflammatory diseases of unknown etiology.
Insights
Substance P (SP) boosts macrophage metabolic activity and enhances pro-inflammatory cytokine release when administered before lipopolysaccharide (LPS). Somatostatin can block this SP effect, highlighting SP's role in immune responses.
Area of Science:
- Immunology
- Neuroscience
- Cell Biology
Background:
- Substance P (SP) is implicated in stress-induced modulation of macrophage function.
- Understanding the direct effects of SP on macrophages in vitro is crucial.
Purpose of the Study:
- To investigate the in vitro effects of substance P (SP) on peritoneal macrophage activity.
- To determine if SP influences metabolic activity and cytokine secretion.
- To examine the role of somatostatin in modulating SP's effects.
Main Methods:
- MTT bioassay to assess cellular metabolic activity.
- Enzyme-linked immunosorbent assay (ELISA) to measure proinflammatory cytokine secretion.
- In vitro incubation of macrophages with SP and lipopolysaccharide (LPS).
Main Results:
- SP significantly increased macrophage metabolic activity.
- Priming macrophages with SP before LPS exposure enhanced proinflammatory cytokine secretion compared to LPS alone.
- Somatostatin antagonized the SP-induced enhancement of cytokine secretion.
Conclusions:
- SP acts as a crucial first signal in macrophage activation cascades.
- The temporal sequence of stimulus administration is critical for in vitro macrophage responses.
- Stress-induced SP release may contribute to the pathogenesis of inflammatory diseases.
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