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A cost effective base-matching assay with low backgrounds
X Su1, G Mushinsky, A M Comeau
1Theobald Smith Research Institute, Inc., The New England Regional Newborn Screening Program, Boston, MA 02130, USA. xsu@world.std.com
Nucleic Acids Research
|November 15, 1996
Abstract:
Base-matching or so-called mini-sequencing is a powerful technique for genotyping and mutation identification. However, its application is often hampered by high background and high cost. We have decreased the background by approximately 5-fold by incorporating an end-blocking step and using only 1/10 of the usual nucleotide concentrations.