Related Experiment Video
Updated: Jul 16, 2026

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Published on: October 10, 2020
An electron spin resonance assay of glutathione S-conjugate transport
Abstract:
A method for studying export of glutathione S-conjugates from cells is proposed based on the use of the spin label tempo-maleimide. This compound is conjugated intracellularly with glutathione and the concentration of the exported conjugate is measured in cell supernatants in an electron spin resonance spectrometer after reoxidation with ferricyanide. This method allows for measurements of micromolar concentrations of the conjugate and requires low amounts of cells.
More Related Videos
07:47Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
03:35Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024