Related Experiment Video
Updated: Aug 2, 2026

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants
Published on: September 12, 2018
Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato
1S. R. Scofield, J. P. Rathjen, J. H. Chang, D. T. Lavelle, NSF Center for Engineering Plants for Resistance Against Pathogens (CEPRAP), University of California, Davis, CA 95616. C. M. Tobias, Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720. R. W. Michelmore, NSF Center for Engineering Plants for Resistance Against Pathogens and Department of Vegetable Crops, University of California, Davis, CA 95616. B. J. Staskawicz, NSF Center for Engineering Plants for Resistance Against Pathogens and Department of Plant and Microbial Biology, University of California, Davis, CA 94720.
Abstract:
Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two-hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.
More Related Videos
09:05Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
06:41High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
Published on: March 10, 2020
Related Concept Videos
Overview of Transposition and Recombination
Gene Regulation in Microbial Communities: Quorum Sensing
Gene Regulation During Sporulation
Modern Molecular Taxonomy
Regulation of Bacterial Virulence