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Related Experiment Videos

Two-component regulatory systems can interact to process multiple environmental signals

F C Soncini1, E A Groisman

  • 1Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

Journal of Bacteriology
|December 1, 1996
PubMed
Summary
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The pleiotropic two-component regulatory system PhoP-PhoQ.

Journal of bacteriology·2001

Salmonella typhimurium uses the PhoP/PhoQ system to control genes responding to magnesium levels. A newly identified PmrA/PmrB system regulates antibiotic resistance and virulence gene expression in response to environmental changes.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • The PhoP/PhoQ two-component system in Salmonella typhimurium regulates numerous genes in response to extracellular magnesium (Mg2+).
  • Understanding bacterial regulatory networks is crucial for deciphering virulence mechanisms and developing novel antimicrobial strategies.

Purpose of the Study:

  • To identify novel Salmonella typhimurium loci regulated by the PhoP/PhoQ system.
  • To characterize the role of the newly identified pmrCAB locus and its regulatory network in bacterial response to environmental stimuli.

Main Methods:

  • Genetic analysis of Salmonella typhimurium strains with mutations in PhoP/PhoQ and PmrA/PmrB systems.
  • Transcriptional analysis of PhoP- and PmrA-regulated genes under varying Mg2+ concentrations and pH conditions.

Related Experiment Videos

  • Identification of the pmrCAB locus as a PhoP-activated gene mediating polymyxin B resistance.

    • Main Results:

    • The pmrCAB locus, encoding the PmrA/PmrB two-component system, was identified as a PhoP-activated locus.
    • PmrA/PmrB regulates genes involved in polymyxin B resistance.
    • PmrA-dependent gene expression is induced by Mg2+ limitation and mild acidification, while PhoP/PhoQ are required for Mg2+-mediated induction but not acid-mediated induction.

    Conclusions:

    • A regulatory cascade involving PhoP/PhoQ and PmrA/PmrB systems allows Salmonella typhimurium to adapt to diverse environmental cues.
    • This intricate regulatory network enables the pathogen to modulate virulence factor expression, enhancing its survival and infectivity.