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A collaborative cytogenetics study to measure and minimize interlaboratory variation

D J Kilian, F M Moreland, M C Benge

    Mutation Research
    |July 1, 1977
    PubMed
    Summary
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    This study standardized rat chromosome aberration analysis across labs, finding triethylenemelamine (TEM) induced significant dose-dependent effects. Rat cytogenetic analysis is effective for mutagenicity testing but requires cautious interpretation.

    Area of Science:

    • Cytogenetics
    • Toxicology
    • Genetics

    Background:

    • Standardized procedures are crucial for reliable interlaboratory results in cytogenetic analysis.
    • Interlaboratory variation can impact the accuracy of mutagenicity testing.
    • Developing uniform protocols minimizes discrepancies in biological assays.

    Purpose of the Study:

    • To assess interlaboratory variation in rat chromosome aberration analysis.
    • To develop standardized methods for minimizing differences in cytogenetic testing.
    • To evaluate the mutagenic potential of triethylenemelamine (TEM) in rats.

    Main Methods:

    • Six cytogenetics laboratories participated in a collaborative study.
    • A joint protocol and common glossary were developed.

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  • Osborne-Mendel rats were exposed to varying doses of TEM (100-400 microgram/kg) or a control.

    • Main Results:

    • Significant dose-dependent clastogenic effects of TEM were observed.
    • Most laboratories demonstrated a consistent dose-response pattern.
    • The proportion of abnormal cells effectively indicated mutagenic potential.

    Conclusions:

    • Rat cytogenetic analysis is a viable system for assessing mutagenic potential.
    • Standardization of protocols enhances the reliability of interlaboratory cytogenetic studies.
    • Caution is advised when interpreting data from specific aberration categories, such as severely damaged cells.