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Related Experiment Videos

Human gingival keratinocytes express E-selectin (CD62E)

E R Pietrzak1, N W Savage, L J Walsh

  • 1Department of Dentistry, University of Queensland, Brisbane, Australia.

Oral Diseases
|March 1, 1996
PubMed
Summary

E-selectin (CD62E) is expressed on gingival keratinocytes, suggesting a role in leukocyte adhesion within the oral epithelium. This finding is crucial for understanding immune cell trafficking in the gingival crevice.

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Area of Science:

  • Immunology
  • Cell Biology
  • Oral Biology

Background:

  • E-selectin (CD62E) is an adhesion molecule mediating leukocyte binding to activated endothelium.
  • Its expression pattern on oral epithelial cells, specifically gingival keratinocytes, was previously uncharacterized.

Purpose of the Study:

  • To characterize E-selectin expression on gingival crevice epithelial cells and oral epithelium.
  • To investigate the potential role of E-selectin in leukocyte-epithelial interactions in the gingiva.

Main Methods:

  • Utilized a panel of six anti-E-selectin monoclonal antibodies.
  • Applied antibodies to cryosections of human gingiva and cultured gingival keratinocytes.

Main Results:

  • Three antibodies (H4/18, H18/7, 1.2B6) showed reactivity with gingival keratinocytes, indicating E-selectin epitope expression.

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  • All six antibodies reacted with endothelial cells; staining with H18/7 and 1.2B6 was weaker and more variable.
  • Exogenous TNF alpha only modestly increased E-selectin expression in cultured keratinocytes.

    • Conclusions:

    • E-selectin epitopes are present on gingival keratinocytes.
    • These epitopes may play a role in leukocyte trafficking within the gingival tissue compartment.