Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

pH-zone-refining countercurrent chromatography

Y Ito1, Y Ma

  • 1Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1676, USA.

Journal of Chromatography. A
|November 8, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute cytogenetic effect of benzene on rat bone marrow cells in vivo and the effect of inducers or inhibitors of drug-metabolizing enzymes.

Mutation research·1992
Same author

[Solitary infected renal cyst: a case report].

Hinyokika kiyo. Acta urologica Japonica·1992
Same author

[Detection of Ureaplasma urealyticum by polymerase chain reaction].

Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases·1992
Same author

Effects of PAF on excitatory neuro-effector transmission in dog airways.

British journal of pharmacology·1992
Same author

Residual disease at the end of induction therapy as a predictor of relapse during therapy in childhood B-lineage acute lymphoblastic leukemia.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·1992
Same author

Risk factors associated with postoperative persistent hypertension in patients with primary aldosteronism.

Surgery·1992
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
Same journal

Ion chromatography: Current strengths, key limitations, and future trends.

Journal of chromatography. A·2026
Same journal

Stereo-sensitive modelling of gas chromatographic retention indices of mono-cycloalkanes in jet fuel range.

Journal of chromatography. A·2026
Same journal

Approaches to using retention indices with coupled column pressure tuning in gas chromatography.

Journal of chromatography. A·2026
Same journal

MOF-supported surface-imprinted polymer for hazard governance of aristolochic acids in herbal matrices: A safety-control strategy supported by multiscale simulations.

Journal of chromatography. A·2026
See all related articles

pH-zone-refining countercurrent chromatography (CCC) is a versatile preparative technique that separates compounds based on pKa and hydrophobicity. This method offers broad applications for various organic molecules and isomers.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Chromatography

Background:

  • Countercurrent chromatography (CCC) is a liquid-liquid separation technique.
  • pH-zone-refining CCC is a specialized preparative technique.
  • This method separates compounds based on their acid-base properties (pKa) and hydrophobicity.

Purpose of the Study:

  • To describe the hydrodynamic mechanism of pH-zone-refining CCC.
  • To illustrate its application in separating diverse organic compounds.
  • To provide technical guidance for optimizing experimental conditions.

Main Methods:

  • pH-zone-refining countercurrent chromatography (CCC).
  • Two elution modes and mathematical analysis.
  • Affinity ligand separations for enantiomers and peptides.

Related Experiment Videos

Main Results:

  • Successful separation of acidic and basic derivatives of amino acids and peptides.
  • Separation of hydroxyxanthene dyes, alkaloids, indole auxins, and isomers.
  • Demonstrated separation of enantiomers, catecholamines, and peptides using affinity ligands.

Conclusions:

  • pH-zone-refining CCC is a powerful and versatile preparative separation technique.
  • The method is applicable to a wide range of organic acids, bases, and isomers.
  • Technical guidance facilitates optimization for various applications, including affinity separations.