[Characteristics of total blood protein measurement during infusion therapy]

The authors have modified R. Flores' method of protein measurements in solutions with bromophenol blue and suggest their modification for measurements of the blood serum total proteins. Experimental and clinical data have confirmed the significant effect of elevated blood levels of glucose and dextrans on the values of blood serum total proteins measured by the common biuret and refractometric methods. The bromophenol blue method is free from these disadvantages. The technique is recommended, first of all, for the infusion therapy departments. The method is highly specific, unsophisticated, and rapid, and is recommended for wide practical use in both inpatient and outpatient clinics. It can be used to measure proteins in other biologic fluids and solutions, provided its levels are not lower than 100 micrograms/ml.