[Effects of incubation temperature on the results of passive hemagglutination test]

Passive hemagglutination test was carried out at several incubation temperatures: 4, 21, 37, and 56 degrees C. Blood sera of donors, salmonellosis enteritidis patients, and specific nonadsorbed sera were used in the study. The least fluctuations of the results were seen in tests with the sera with the least manifest immune properties. The optimal incubation temperatures were found 4 and 21 degrees C, and in 1/3 of cases cold incubation was found preferable. The relationship between antibody titers and incubation temperature may be described by the formula: T4 > or = T21 > or = T37 > or = T56, with Tn representing the reverse value of antibody titer in certain incubation temperature.