Natural anthracenone subcellular distribution and effects on NADPH-cytochrome P450 reductase microsomal activity

Natural anthracenone subcellular distribution and effects on NADPH-cytochrome P450 reductase microsomal activity. Subcellular distribution study of a natural anthracenone (T-514) isolated from Karwinskia humboldtiana showed to be homogeneous on subcellular (nuclear, mitochondrial, peroxisomal and microsomal) fractions prepared from rat liver treated with an acute dose of T-514. These results indicate that T-514 can pass easily through subcellular compartment membranes and an absence of selectivity for some subcellular organelles. A significant increase of protein on liver homogenates and NADPH-cytochrome P450 reductase microsomal activity indicates that T-514 may act as a microsomal enzymatic inducer. In addition, this enzymatic specific activity increment could be due to the interaction of T-514 with the microsomal redox cycling.