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PCR cloning of the cDNA encoding baboon L-selectin
1Protein Design Labs, Inc., Mountain View, CA 94043, USA. naova@pdl.com
Gene
|November 28, 1996
Summary
Researchers isolated baboon L-selectin cDNA from lymphocytes, determining its nucleotide sequence. The resulting 372-amino acid protein sequence shows 95% identity to human L-selectin, aiding comparative immunology studies.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- L-selectin plays a crucial role in lymphocyte homing and immune surveillance.
- Understanding L-selectin in non-human primates can provide insights into human immune responses.
Purpose of the Study:
- To isolate and characterize the baboon L-selectin cDNA.
- To determine the amino acid sequence of baboon L-selectin.
- To compare baboon L-selectin with its human counterpart.
Main Methods:
- Isolation of cDNA from baboon peripheral blood lymphocytes.
- Nucleotide sequencing of the isolated cDNA.
- Deduction of the amino acid sequence.
Main Results:
- Successfully isolated baboon L-selectin cDNA.
- Determined the nucleotide sequence.
- The deduced 372-amino acid sequence of baboon L-selectin was obtained.
- Baboon L-selectin shares 95% amino acid identity with human L-selectin.
Conclusions:
- The high sequence identity suggests conserved function between baboon and human L-selectin.
- This finding supports the use of baboons as a model for studying L-selectin-mediated immune processes.