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[Storage-induced rheologic and biochemical changes in erythrocyte concentrates with added solution and possible
B Mansouri Taleghani1, R Grossmann, G Waltenberger
1Abteilung für Transfusionsmedizin und Immunhämatologie in der Chirurgischen Universitätsklinik, Würzburg.
Beitrage Zur Infusionstherapie Und Transfusionsmedizin = Contributions to Infusion Therapy and Transfusion Medicine
|January 1, 1996
Summary
Packed red blood cells (prbc) stored in SAG-M show time-dependent declines in rheological properties, including increased viscosity and filter clogging. These changes correlate with biochemical alterations, highlighting the need for rheological assessments in quality control.
Area of Science:
- Hematology
- Biophysics
- Blood banking
Context:
- Red blood cell (RBC) rheological integrity is crucial for capillary circulation.
- Existing research extensively covers metabolic/biochemical changes in RBCs but lacks studies on packed RBCs (prBC) in additive solutions regarding quality.
- Standard operating procedures (SOP) were followed to prepare prBC in SAG-M and plasma from 16 donations, with buffy coats removed.
Purpose:
- To investigate the time-dependent changes in rheological properties of prBC stored in SAG-M.
- To correlate these rheological alterations with biochemical changes (pH, ATP, 2,3-DPG) during storage.
- To evaluate prBC viscosity, filterability, aggregability, and filter clogging rate over 28 days.
Summary:
- Measurements included extracellular pH, ATP, and 2,3-DPG content, alongside prBC viscosity, filterability, aggregability, and filter clogging rate on days 1, 14, and 28.
- Results confirmed a time-dependent deterioration of prBC rheology in SAG-M, characterized by increased viscosity and filter clogging, and decreased filterability and aggregability.
- Biochemical analysis showed expected decreases in pH, ATP, and 2,3-DPG during storage, consistent with known literature on RBC flexibility and shape changes.
Impact:
- The findings demonstrate a clear link between biochemical degradation and impaired rheological function of stored prBC.
- This study underscores the importance of rheological assessments as a complementary measure to biochemical analyses for comprehensive quality assurance of prBC.
- Recommendations include incorporating rheological examinations for evaluating new additive solutions or significant SOP modifications.