[Inline filtration of erythrocyte concentrates with the Leucoflex LCR4 T/B system]

The in-line-filtration of red cell concentrates (RCC) is an effective method to reduce white-cell-induced reactions. SAG-M RCCs were prepared in quadruple top/bottom bag systems and filtered after storage at 4 degrees C (study: n = 12). The mean white blood cell (WBC) reduction was 99.9% with the number of residual WBCs at about 0.67 x 10(6) per unit and with a loss of Hb mass of 10.64%. No cell fragments of WBCs and monocytes were detected (MAIPA). After storage of 42 days, mean values of hemolysis level (0.64%) and ATP concentration (2.27 mumol/g Hb) were improved compared with unfiltered RCCs. Comparably good results of leukocyte depletion were achieved by preparation of about 3,000 in-line filtrated RCCs for clinical use, measured by routine quality control (n = 25). Our experience shows that the systems are easy to handle and to use. The Leucoflex LCR4 in-line T/B system (Maco Pharma) can be recommended in the clinical practice by virtue of the effective leukocyte elimination, particularly in those blood banks with platelet concentrate production from buffy coats beside the RCC in-line filtration.