Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse polymorphonuclear granulocyte binding to extracellular matrix molecules involves beta 1 integrins

M Frieser1, R Hallmann, S Johansson

  • 1Institute for Experimental Medicine, University of Erlangen-Nürnberg, Germany.

European Journal of Immunology
|December 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deciphering the heterogeneity of the Lyve1<sup>+</sup> perivascular macrophages in the mouse brain.

Nature communications·2022
Same author

The gelatinases, MMP-2 and MMP-9, as fine tuners of neuroinflammatory processes.

Matrix biology : journal of the International Society for Matrix Biology·2017
Same author

Postinterventional pain and complications of sonographically guided interventions in the liver and pancreas.

Ultraschall in der Medizin (Stuttgart, Germany : 1980)·2013
Same author

GDF-15 prevents platelet integrin activation and thrombus formation.

Journal of thrombosis and haemostasis : JTH·2012
Same author

Efficacy of contrast-enhanced US versus CT or MRI for the therapeutic control of percutaneous radiofrequency ablation in the case of hepatic malignancies.

Ultraschall in der Medizin (Stuttgart, Germany : 1980)·2011
Same author

Quantitative lymphatic vessel trait analysis suggests Vcam1 as candidate modifier gene of inflammatory bowel disease.

Genes and immunity·2010
Same journal

Fast Generation of F(Ab')<sub>2</sub> Fragments From Human IgG Using Fc-Fused IgG-Degrading Enzyme.

European journal of immunology·2026
Same journal

Generation of Regulatory T Cells Against Islet Neoantigen.

European journal of immunology·2026
Same journal

Complement Inhibition in the Clinic: Are We Doing Enough to Protect Patients From Infection?

European journal of immunology·2026
Same journal

Special Issue: yEFIS 3rd Symposium.

European journal of immunology·2026
Same journal

CRISPR/Cas9-Mediated Gene Knockout Reveals a Nonredundant Role for p16<sup>INK4A</sup> in Controlling TCR-Dependent and Independent CD8 T Cell Expansion.

European journal of immunology·2026
Same journal

Induction of Humoral and Cellular Immunity After SARS-CoV-2 JN.1 Vaccination in Individuals With and Without Prior Infection.

European journal of immunology·2026
See all related articles

Mouse immune cells (PMN) and cell lines (32DC13, WEHI 78/24) show specific adhesion to fibronectin and vitronectin, mediated by integrins alpha5 beta1 and alpha(v) beta3, respectively. They do not bind basement membrane proteins, impacting leukocyte extravasation.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Leukocyte adhesion to extracellular matrix (ECM) is crucial for inflammatory and immune responses.
  • Understanding the specific integrin-mediated interactions of different leukocyte types with ECM proteins is vital for deciphering cell migration mechanisms.

Purpose of the Study:

  • To investigate and compare the adhesion mechanisms of mouse polymorphonuclear granulocytes (PMN), 32DC13, and WEHI 78/24 cell lines to various ECM proteins.
  • To identify the specific integrins involved in these adhesion processes.
  • To relate these findings to leukocyte extravasation.

Main Methods:

  • Adhesion assays were performed using purified mouse PMN, 32DC13, and WEHI 78/24 cell lines.
  • Cells were tested for binding to fibronectin, vitronectin, laminin-1, collagen type IV, and perlecan under varying activation states.

Related Experiment Videos

  • Inhibitory peptides (RGDfV) and antibodies were used to determine the integrin mechanisms involved.

    • Main Results:

    • All three cell types exhibited specific binding to fibronectin and vitronectin, with varying affinities and responses to activation (PMA).
    • 32DC13 cells showed significant binding to laminin-1, mediated by alpha6 beta1 integrin.
    • PMN and WEHI 78/24 cells primarily adhered to fibronectin (via alpha5 beta1) and vitronectin (via alpha(v) beta3), with limited changes upon activation.
    • None of the cell types bound to collagen type IV or perlecan; these proteins appeared to repel the cells.

    Conclusions:

    • Mouse PMN and tested cell lines utilize specific integrins (alpha5 beta1, alpha(v) beta3) for adhesion to interstitial ECM proteins like fibronectin and vitronectin.
    • The limited binding to basement membrane proteins suggests a role in regulating leukocyte extravasation.
    • These findings highlight differential integrin usage and ECM interactions relevant to leukocyte trafficking in inflammatory processes.