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Novel agglutinin in the midgut of the tick Ixodes ricinus
J Uhlír1, L Grubhoffer, P Volf
1Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceské Budĕjovice, Czech Republic.
Folia Parasitologica
|January 1, 1996
Abstract:
Haemagglutination activity (HA) was found and characterized in a midgut homogenate of Ixodes ricinus (L.). HA was induced by tick feeding; it was not detected in starved ticks. In a haemagglutination inhibition test, HA showed an affinity for some carbohydrates (N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, rhamnose, and dulcit) and glycoconjugates (especially lipopolysaccharides). Midgut protein components of 37, 60, 65, and 73 kDa were identified by immunoblotting as potential structural subunits of the new agglutinin.