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Pooled human immunoglobulins contain anti-idiotypes with reactivity against the SLE-associated 4B4 cross-reactive
F DeKeyser1, H DeKeyser, M D Kazatchkine
1University of Texas Health Science Center, Department of Medicine, San Antonio, USA.
Objective:
To identify anti-idiotype antibody activity against a regulatory anti-Sm idiotype (4B4) in pooled human IgG (IVIG).
Methods:
ELISA were used to determine anti-idiotype antibody activity in IVIG.
Results:
Anti-idiotype antibody activity to 4B4 was detected in IVIG. The anti-idiotypic activity in IVIG could be inhibited by antigen, Sm, and rabbit anti-idiotypic antiserum. The idiotypic binding activity was predominately directed against the 4B4 VH region.
Conclusion:
IVIG contains antibodies against a lupus-associated crossreactive idiotype. These antibodies represent Ab2 beta type antibodies. The anti-idiotypic activity in IVIG may be relevant to the efficacious use of IVIG in treating rheumatic diseases.