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Structural basis for the catalytic mechanism and substrate specificity of the ribonuclease alpha-sarcin
R Campos-Olivas1, M Bruix, J Santoro
1Instituto de Estructura de la Materia, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
FEBS Letters
|December 9, 1996
Abstract:
alpha-Sarcin is a ribosome-inactivating protein which selectively cleaves a single phosphodiester bond in a universally conserved sequence of the major rRNA. The solution structure of a-sarcin has been determined on the basis of 1898 distance and angular experimental constraints from NMR spectroscopy. It reveals a catalytic mechanism analogous to that of the T1 family of ribonucleases while its exquisite specificity resides in the contacts provided by its distinctive loops.