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Rosetteness in Portulaca grandiflora: an altered genetic expression
P Venu-Babu1, V K Ogale, S D Mishra
1Molecular Biology and Agriculture Division, B.A.R.C., Trombay, Bombay, India.
Molecular Biology Reports
|January 1, 1996
Summary
Prolonged growth or pruning induces rosetteness in Portulaca grandiflora. Stem protein analysis revealed a 58 kDa polypeptide and altered lower molecular weight proteins, but leaf protein profiles remained unchanged.
Area of Science:
- Plant physiology
- Molecular biology
- Biochemistry
Background:
- Rosetteness, a plant growth abnormality, can be triggered by environmental factors or developmental processes.
- Portulaca grandiflora exhibits rosetteness, particularly under conditions of extended vegetative growth or pruning.
Purpose of the Study:
- To investigate the molecular changes associated with stress-induced rosetteness in Portulaca grandiflora.
- To identify specific protein alterations in stems and leaves of plants exhibiting rosetteness.
Main Methods:
- Analysis of cellular extracts from Portulaca grandiflora stems and leaves.
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to compare protein profiles.
Main Results:
- SDS-PAGE revealed the induction of a high molecular mass polypeptide (approximately 58 kDa) in rosette stems.
- Over-expression of several lower molecular weight polypeptides was observed in rosette stems.
- No significant differences in protein profiles were detected in the leaves of affected plants.
Conclusions:
- Prolonged vegetative growth and pruning are identified as potential inducers of rosetteness in Portulaca grandiflora.
- Specific protein changes in the stems, including a novel 58 kDa polypeptide, are associated with rosetteness.
- Leaf tissues do not exhibit comparable protein alterations, suggesting localized stem responses.