[The morphological differentiation of murine neuroblastoma NIE-115 cells]

Characters and kinesis of mouse neuroblastoma (MN) cells morphological differentiation affected by non-serum, hypo- and hyperosmotic media and salt solution were studied. The nature of morphological differentiation was found not to depend on the inductors, while kinesis varies significantly. Morphological differentiation speed is directly proportional to the extent of non-specific action and probability of the following cell death. on the other hand, the number of irreversibly differentiated cells is inversely proportional to the action strength. For the induction of morphological differentiation a minimal nutrition media do not containing serum is sufficient and a common growth media changed once in 3-5 days according to how it acidifies is better to use for the prolonged maintenance of it. Universality of neuronal morphological differentiation is under discussion according tot he data obtained from MN cells and cultured mollusk isolated neurons.