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[Ultrastructure of Mycoplasma sp. demonstrated by phosphotungstic acid-chromic acid pH 0,4]
Abstract:
Ultrastructural observation of Mycoplasma sp. revealed by the phosphotungstic acid-cromic acid, pH 0,4. Arq. Inst. Biol., São Paulo, 45(3) :169-176, 1978. Cells of IB-RS-2 (clone C-26-3) swine line were examined under electron microscopy, employing the differential coloration with PTA-ACr 5 %, pH 0,4, during 30 minutes, at 37 degrees C. Through this method we could observe the intracitoplasmatic forms, electronically dense, rather pleomorfic, with sizes varying between 60nm and 640nm and a unitary membrane of 90A similar to the extracellular forms described as Mycoplasma sp.
Insights
Ultrastructural analysis revealed Mycoplasma sp. forms within swine cells using phosphotungstic acid-chromic acid staining. This method visualized pleomorphic intracellular structures resembling extracellular Mycoplasma sp.
Area of Science:
- Microbiology
- Cell Biology
- Microscopy
Context:
- Swine cell lines (IB-RS-2) were utilized for ultrastructural examination.
- Mycoplasma sp. identification and characterization are crucial in veterinary diagnostics.
- Electron microscopy provides high-resolution imaging of microbial structures.
Purpose:
- To investigate the ultrastructure of Mycoplasma sp. within host cells.
- To evaluate the efficacy of phosphotungstic acid-chromic acid (PTA-ACr) staining for visualizing intracellular Mycoplasma.
- To compare intracellular forms with known extracellular Mycoplasma sp. morphology.
Summary:
- Electron microscopy was employed on IB-RS-2 swine cells using PTA-ACr 5% staining at pH 0.4.
- Intracitoplasmatic, pleomorphic forms of Mycoplasma sp. were observed, ranging from 60nm to 640nm.
- These intracellular forms exhibited a unitary membrane (90Å) and resembled extracellular Mycoplasma sp.
Impact:
- This study demonstrates a method for visualizing intracellular Mycoplasma sp. in host cells.
- The findings contribute to understanding the morphology and potential intracellular presence of Mycoplasma sp.
- Enhanced diagnostic capabilities for Mycoplasma infections in swine may be developed based on these ultrastructural observations.