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Related Experiment Videos

DNA sequences of RAPD fragments in artiodactyls

S Kostia1, J Palo, S L Varvio

  • 1Department of Biosciences, University of Helsinki, Finland.

Genome
|April 1, 1996
PubMed
Summary

Researchers sequenced bovine DNA fragments from a random amplified polymorphic DNA (RAPD) profile. Three fragments showed homology to mammalian genes, including a human inositol trisphosphate receptor gene, with variations in microsatellite repeats observed in related species.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Comparative Genomics

Background:

  • Random Amplified Polymorphic DNA (RAPD) analysis is a molecular technique used to detect polymorphisms in DNA.
  • Sequencing of amplified DNA fragments provides insights into genetic composition and evolutionary relationships.
  • Mammalian genomes contain repetitive elements and genes with conserved functions across species.

Purpose of the Study:

  • To characterize bovine DNA sequences obtained from RAPD analysis.
  • To identify homologies of these sequences with known mammalian genes and repetitive elements.
  • To investigate the genetic basis of size variation in homologous fragments across different cervid species.

Main Methods:

  • Generation of a bovine RAPD profile using a 10-mer primer.

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  • Isolation and sequencing of major DNA fragments from the RAPD profile.
  • Bioinformatic analysis to determine sequence homology with existing databases (e.g., mammalian sequences, human genes).

    • Main Results:

    • Three out of four sequenced bovine DNA fragments exhibited significant homology to known mammalian sequences.
    • Identified homologies include sequences related to LINE elements (61-66% identity) and a human chromosome 2 sequence tagged site (78.5% identity).
    • A high degree of identity (93.1%) was found with the human type 2 inositol 1,4,5-trisphosphate receptor gene; homologous fragments in white-tailed deer and reindeer showed size variations attributed to microsatellite repeat number differences.

    Conclusions:

    • Bovine RAPD fragments can represent conserved mammalian genes and repetitive elements.
    • The identified human inositol trisphosphate receptor gene homologue is conserved across cervid species, with size variation linked to microsatellite repeat polymorphisms.
    • This study highlights the utility of RAPD sequencing for identifying functionally relevant genetic markers and understanding evolutionary patterns in mammals.