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Related Experiment Videos

B-CLL cells with unusual properties

J Avila-Cariño1, N Lewin, Y Tomita

  • 1Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden. Javier.Avila-Carino@mtc.ki.se

International Journal of Cancer
|January 6, 1997
PubMed
Summary
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This study details a unique case of chronic lymphocytic leukemia (CLL) with T-cell markers and a specific translocation. These CLL cells were susceptible to Epstein-Barr virus (EBV) infection in vitro, suggesting a potential in vivo T-cell response eliminated them.

Area of Science:

  • Hematology
  • Virology
  • Immunology

Background:

  • Chronic lymphocytic leukemia (CLL) typically expresses B-cell markers.
  • Epstein-Barr virus (EBV) is known to infect B cells.

Purpose of the Study:

  • To investigate the unusual properties of CLL cells from a patient with T-cell markers and a specific translocation.
  • To explore the interaction between these unique CLL cells and Epstein-Barr virus (EBV) in vitro.
  • To understand the potential in vivo immune response to such aberrant cells.

Main Methods:

  • Characterization of CLL cells for B-cell and T-cell markers (CD3, CD8).
  • Karyotyping to identify chromosomal translocations, specifically t(18;22)(q21;q11) involving bcl-2 and Ig lambda.
  • In vitro infection and immortalization of CLL cells with EBV.

Related Experiment Videos

  • Detection of EBV nuclear antigen (EBNA) in ex vivo and in vitro samples.
  • Generation of cytotoxic T-lymphocyte responses against autologous EBV-infected CLL cells.

    • Main Results:

    • CLL cells expressed both B-cell and T-cell markers (CD3, CD8) and had a t(18;22)(q21;q11) translocation, leading to high bcl-2 expression.
    • These CLL cells were susceptible to EBV infection and immortalization in vitro, forming established cell lines.
    • T-cell markers were lost in the immortalized EBV-infected CLL lines.
    • No EBNA-positive cells were detected in the patient's ex vivo blood sample, despite in vitro sensitivity.
    • Autologous T-lymphocytes demonstrated cytotoxic function against EBV-infected CLL cells in vitro.

    Conclusions:

    • The unique CLL cells with T-cell markers and bcl-2/Ig lambda translocation are highly susceptible to EBV infection and immortalization.
    • The absence of EBNA-positive cells in vivo suggests that an immune surveillance mechanism, likely a T-cell response, eliminates these aberrant cells.
    • This case highlights the potential for T-cell mediated elimination of malignant B cells upon viral infection.