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Expression of two cDNAs encoding class I chitinases of rice in Escherichia coli
1Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
Bioscience, Biotechnology, and Biochemistry
|August 1, 1996
Abstract:
Two cDNAs encoding class I chitinases of rice were expressed in Escherichia coli. The cDNAs were fused to the MS2-polymerase gene in an expression vector, pEx31. The fusion proteins, expressed under the control of the lambda PL-promoter, showed the chitinase activity independent of the existence of the hevein domain. The enzymatic hydrolysis of colloidal chitin by the fusion proteins showed that the proteins were endo-type enzymes.