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Updated: Oct 4, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Stereoconfiguration of anteiso-fatty biosynthesized from DL-isoleucine in rat skin
1Laboratory of Applied Biochemistry, Faculty of Agriculture, University of Ryukyus, Okinawa, Japan.
Abstract:
Isoleucine (Ile) is a precursor for the biosynthesis of anteiso-fatty acids in rat skin, and among the four possible stereoisomers of Ile, L-Ile, and L-allo-Ile were selectively used for biosynthesis of anteiso-fatty acids. This study examined the optical rotation of anteiso-fatty acid derived from DL-Ile to ascertain its stereoconfiguration. Specific rotation of anteiso-fatty acid derived from DL-Ile favorably compared with that derived from L-Ile, suggesting the selective biosynthesis of the (S)-enantiomer of anteiso-fatty acid in rat skin.
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