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Updated: Aug 6, 2026

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
Published on: January 7, 2022
In vitro systems in the study of peroxisomal protein import
1Centre for Plant Biochemistry and Biotechnology, University of Leeds, United Kingdom. a.baker@leeds.ac.uk
Abstract:
Our level of understanding of peroxisome biogenesis in comparison with other cellular organelles is rudimentary, yet the fragments of information available indicate that the targeting and import of peroxisomal proteins occur by fundamentally different mechanisms. Genetic studies have identified a number of genes required for peroxisome assembly, but in most cases the functions of the gene products remain unknown. In vitro protein translocation systems have played a prominent role in unravelling the biochemistry of protein translocation into other organelles. This review considers some of the requirements for establishing a bona fide peroxisomal import assay and discusses the findings which have emerged as a result of using such experimental systems.
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