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Properties of the regulatory subunit of cAMP-dependent protein kinase type II from human brain
I D Grozdova1, N A Alexandrova, E V Sveshnikova
1Russian Research Center of Molecular Diagnostics and Therapy, Moscow, Russia.
Biochemistry and Molecular Biology International
|December 1, 1996
Abstract:
The regulatory subunit type II (RII) of cAMP-dependent protein kinase purified from human brain was represented by two proteins with apparent molecular masses of 51-52 kD and 54 kD. Dephosphorylation of human RII containing 3 mol phosphate/mol protein did not change the electrophoretic pattern. One-dimensional peptide mapping of 51-52 kD and 54 kD proteins after digestion with St. aureus V8 protease evidenced to their being distinct proteins. The data obtained permit to assume that human RII of neural type is represented by two isoforms.