Complete coding region of the new HLA-DQB1*0612 allele, obtained by RT-PCR
C Vilches1, J M García-Pacheco, R de Pablo
1Servicio de immunologia, Clinica Puerta de Hierro, Madrid, Spain.
Tissue Antigens
|November 1, 1996
Abstract:
A novel HLA-DQB1 allele was detected by oligotyping in the Bubi population of Equatorial Guinea. In order to characterize the new allelic variant, a RT-PCR method which permitted the cloning of its complete coding region was designed. With this method, we have determined the nucleotide sequence of the new DQB1*0612 allele, related to *0604 and *0609 but differing from them at polymorphic codon 70. A proposal for the improvement of the sequencing strategies of HLA class II alleles is made.
