Local cell traffic and cytokine production associated with ectoparasite infection

This paper reviews recent advances in our understanding of changes in local cellular traffic and cytokine synthesis that occur as a result of infection of sheep with the ectoparasite Lucilia cuprina. Changes in the cellular composition and cytokine profile of infected skin and draining afferent and efferent lymph were assessed using standard approaches and, in addition, a variety of techniques that have only recently become available as a result of advances in ruminant cytokine biology. These include cytokine-specific immunoassay, reverse transcription PCR (RT-PCR) and immunohistology. The initial acute inflammatory response was characterised by the infiltration of polymorphonuclear cells followed by selected lymphocyte subsets into discrete areas adjacent to the site of infection. Analysis of cytokine expression in skin prior to and following infection provided a molecular basis for the observed cellular events. Both cellular and molecular events within the skin were reflected within draining afferent lymph providing a basis for the conclusion that events within the skin (other than antigen uptake and transport) may influence events within the draining node and thus the outcome of the immune response to the parasite. Analysis of cellular and molecular changes in efferent lymph during infection suggested initiation of antigen-specific immunity.