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Related Experiment Videos

B-cell-derived granulocyte-colony stimulating factor (G-CSF)

A Corcione1, V Pistoia

  • 1Laboratory of Oncology, Scientific Institute G. Gaslini, Genoa, Italy.

Methods (San Diego, Calif.)
|January 1, 1997
PubMed
Summary

Granulocyte-colony stimulating factor (G-CSF) is produced by activated B lymphocytes, particularly germinal center B cells. This cytokine enhances B-cell survival and may play a role in B-cell malignancies.

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Area of Science:

  • Immunology
  • Hematology

Background:

  • B lymphocytes, specifically germinal center (GC) B cells, are known to be activated in vivo.
  • Granulocyte-colony stimulating factor (G-CSF) is a cytokine with known biological activity.

Purpose of the Study:

  • To investigate the production of G-CSF by normal and neoplastic human B lymphocytes.
  • To determine the role of G-CSF in B-cell survival and apoptosis.

Main Methods:

  • Culture of tonsillar B lymphocytes and neoplastic B cells from lymphoma and chronic lymphocytic leukemia (CLL) patients.
  • Measurement of G-CSF release in culture supernatants.
  • Assessment of B-cell apoptosis and survival in response to G-CSF and other stimuli.

Main Results:

  • Activated tonsillar B lymphocytes, especially GC B cells, spontaneously released G-CSF. Mantle zone B cells did not produce G-CSF.
  • CD40 monoclonal antibody (mAb) and recombinant interleukin-4 (rIL4) increased G-CSF production by GC B cells, correlating with enhanced cell survival.
  • Neoplastic B cells from follicular center cell lymphoma released G-CSF spontaneously, while a subset of B-CLL cells required stimulation for G-CSF production.
  • Recombinant G-CSF (rG-CSF) rescued both normal GC B cells and some B-CLL cells from apoptosis.

Conclusions:

  • G-CSF is produced by normal and neoplastic human B lymphocytes.
  • G-CSF modulates apoptosis in B lymphocytes, suggesting autocrine and/or paracrine functions.
  • G-CSF may be involved in the pathogenesis or survival of B-cell malignancies.

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