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[The microtrephine: experimental experiences, initial clinical application]

N F Schrage1, M Reim

  • 1Augenklinik RWTH Aachen.

Klinische Monatsblatter Fur Augenheilkunde
|August 1, 1996
PubMed
Summary
This summary is machine-generated.

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A novel microtrephine enables precise corneal stromal biopsies for disease diagnosis. This minimally invasive technique is safe and repeatable, allowing for early detection and comprehensive analysis of corneal conditions.

Area of Science:

  • Ophthalmology
  • Surgical Innovation
  • Diagnostic Pathology

Context:

  • Corneal diseases necessitate stromal tissue evaluation for accurate diagnosis and treatment.
  • Existing biopsy methods often cause significant corneal damage, hindering therapeutic goals.
  • A need exists for a clinically applicable, minimally invasive corneal biopsy technique.

Purpose:

  • To develop and evaluate a new microtrephine instrument for obtaining corneal biopsies.
  • To assess the safety, efficacy, and precision of the microtrephine in collecting stromal and epithelial samples.

Summary:

  • A 160-micron rotating microtrephine was developed for corneal biopsies, yielding solid cylinders of epithelium and stroma.
  • The procedure was tested on rabbit and human corneas, demonstrating a low perforation rate (0.63% in rabbits, 0% in humans).

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  • Successful biopsies were obtained in 75% of cases, enabling various microscopic and microbiological analyses, including PCR for Herpes Simplex Virus.

    • Impact:

    • Enables precise, repeatable stromal biopsies with minimal corneal damage.
    • Facilitates early bioptic diagnostics for a wide range of corneal diseases.
    • Supports comprehensive routine diagnostics, including repeated sampling from a single cornea.