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Related Experiment Videos

Normal values for the nitroblue tetrazolium test (NBT. test)

O Okong'o, K A Fernando, G G Rwegellera

    Medical Journal of Zambia
    |June 1, 1977
    PubMed
    Summary

    The Nitroblue Tetrazolium (NBT) test measures neutrophil function. Results showed a logarithmic decrease in positive cells over time, enabling a new normogram for interpreting stored blood samples.

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    Area of Science:

    • Immunology
    • Hematology
    • Clinical Diagnostics

    Background:

    • The Nitroblue Tetrazolium (NBT) test is a diagnostic assay assessing neutrophil oxidative burst activity.
    • Established NBT test values serve as a benchmark for evaluating neutrophil function across different populations.
    • Interpreting NBT results from stored blood samples presents challenges due to potential changes over time.

    Purpose of the Study:

    • To quantify the mean count of positive neutrophils in the NBT test.
    • To investigate the effect of time on NBT test results in blood samples.
    • To establish a basis for a normogram to interpret NBT results from stored blood.

    Main Methods:

    • Neutrophil counts were determined using the NBT test.
    • Neutrophils were stimulated with heat-killed bacteria to assess response.
    • The number of positive cells was measured over time.

    Main Results:

    • The mean count of positive neutrophils without stimulation was 10.9 ± 1.2 SE.
    • Neutrophils stimulated with heat-killed bacteria showed a mean positive count of 51.6 ± 2.2 SE.
    • A logarithmic decrease in the number of positive NBT cells was observed over time.

    Conclusions:

    • The NBT test results are comparable to previously reported values.
    • The time-dependent logarithmic decrease in positive cells allows for the development of a normogram.
    • This normogram will aid in the accurate interpretation of NBT test results from stored blood samples.

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