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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Methods and applications of three-color confocal imaging

S W Paddock1, E J Hazen, P J De Vries

  • 1Howard Hughes Medical Institute, Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA. paddock@facstaff.wisc.edu

Biotechniques
|January 1, 1997
PubMed
Summary

This study presents a straightforward Adobe Photoshop method for merging grayscale confocal fluorescence images into two- and three-color composite images. It explores various computer-based image manipulation techniques relevant to multi-label confocal microscopy in biomedical research.

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