Production of monospecific antisera against two vaccinia virus antigens

Vaccinia virus antigen was prepared from infected rabbit lung fibroblast monolayers grown in Eagle's medium supplemented with serum obtained from the rabbit going to be used for immunization. Monospecific rabbit antisera against the L and S antigens were produced by immunization with immunoprecipitates cut out from the agarose after quantitative line immunoelectrophoresis using antigen produced in HeLa cells.