Determination of Photosan III in human plasma

In order to develop an accurate and quick method for the determination of Photosan III in human plasma, we used statistically planned experiments with an aim to identify the factors that can influence the analysis. Through a series of 20 experiments based on acid extraction of the porphyrin from the plasma and subsequent fluorescence analysis a calibration was obtained between 0 and 3.3 x 10(-5) M. The deviation of the parameters around the regression line is 3.02%, the coefficient of variation 3.55%.